Intraoral wound closure with tissue-engineered mucosa: new perspectives for urethra reconstruction with buccal mucosa grafts.

摘要: In urethra reconstruction, the creation of a new from free oral mucosa graft is an established surgical technique. The removed at same time that reconstruction procedure performed. Depending on size required, intraoral wound closed primarily or left to heal secondarily. latter method limits this technique by leading scars strictures, which have negative impact condition soft tissue. Therefore, in study, pilot study involving 12 patients, tissue-engineered was tested for covering defects avoid drawbacks mentioned above. For tissue-graft engineering, biopsy sample 2 4 mm diameter hard palate approximately weeks before be addition, 30 ml autogenous serum extracted venous whole-blood sample. primary cultures were incubated Dulbecco modified Eagle's medium and nutrient factor F (Gibco Co., Eggenstein, Germany), containing usual additives serum. After period 3 weeks, subcultivation performed engineer transplants consisting several layers keratinocytes support foil. thorough intraoperative blood coagulation had occurred, cultured carrier foil applied surface fixed single sutures. Additionally, covered 8 10 days dressing, also onto suture loops. It possible perform closure with cover defect sizes as large 11.0 x 4.0 cm. This provides better prospect both tissue defects. number strictures are diminished. special interest functional unit cavity, including cosmetic conditions (e.g., case prosthetic rehabilitation). comparison local tissue, presented reduces severity postoperative pain allows faster rehabilitation patients because wound-healing process. Furthermore, mobility structures achieved.