In vivo behavior of complete human oral mucosa equivalents: characterization in athymic mice

摘要: Pena I, Junquera LM, Meana A, Garcia E, Aguilar C, Fresno MF. In vivo behavior of complete human oral mucosa equivalents: characterization in athymic mice. J Periodont Res 2011; 46: 214–220. © 2010 John Wiley & Sons A/S Background and Objective:  The interest tissue engineering as a way to achieve repair damaged body tissues has led the carrying out many studies whose results point potential effectiveness these methods. previous study, we reported obtaining autologous equivalents (CAOMEs), characterized by immature keratinocytes stem cells on an plasma fibroblast scaffold. purpose this study is show their vivo, using them free grafts experimental animals, demonstrate capacity regenerate mucosa. Material Methods:  We engineered CAOMEs, previously described. All CAOMEs thus obtained were used nu/nu To assess evolution studied histological immunohistochemical features AE1/AE3 pancytokeratin, 5/6 cytokeratin pair, cytokeratin 13, laminin 5, collagen IV, vimentin, p-63 Ki-67, at 7, 14 21 d. Results:  structure became progressively closer that samples. Cytokeratin 5/6 staining increasingly intense basal suprabasal layers, cytokeratin 13 was exclusively positive superficial layers. membrane completed 21 d. Vimentin showed correct formation chorion. Ki-67 indicated regeneration process taking place. Conclusion:  present shows regenerative ability reach maturity similar seen mucosa.