Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus.

摘要: BACKGROUND Circulating microRNAs (miRNAs) are potential biomarkers for many diseases. However, they can originate from non-disease specific sources, such as blood cells, and compromise the investigations miRNA biomarkers. While small extracellular vesicles (sEVs) have been suggested to provide a purer source of circulating miRNAs discovery, most suitable sample sEV biomarker studies has not defined. AIM To compare profiles between matched serum plasma preparations determine their suitability studies. METHODS Matched samples were obtained 10 healthy controls patients with esophageal adenocarcinoma. isolates prepared using ExoQuickTM quantified NanoSight. RNA was extracted miRNeasy Serum/Plasma kit profiled Taqman Openarray qPCR. The overall content expression reported vesicular non-vesicular origins compared preparations. diagnostic performance previously identified multi-miRNA panel adenocarcinoma also compared. RESULTS higher in (480 miRNAs) contained 97.5% found (412 miRNAs).The commonly expressed highly correlated (Spearman's R = 0.87, P < 0.0001) preparations, but consistently Specific blood-cell (hsa-miR-223-3p, hsa-miR-451a, miR-19b-3p, hsa-miR-17-5p, hsa-miR-30b-5p, hsa-miR-106a-5p, hsa-miR-150-5p hsa-miR-92a-3p) at 2.7 9.6 fold levels (P 0.05). In percentage protein-associated relatively (Ct 20-25) greater than (50% vs 31%). vesicle-associated (70% 44%). A 5-miRNA produced cross validated accuracy discriminating (AUROC 0.80 0.54, CONCLUSION Although more non-vesicle origins. Serum appears be