Capillary electrophoresis/electrospray ion trap mass spectrometry for the analysis of negatively charged derivatized and underivatized glycans

摘要: The increasing interest in the development of glycoproteins for therapeutic purposes has created a greater demand methods to characterize sugar moieties bound them. Traditionally, released carbohydrates are derivatized using such as permethylation or fluorescent tagging prior analysis by high performance liquid chromatography (HPLC), capillary electrophoresis (CE), direct infusion mass spectrometry. However, little research been performed CE with on-line spectrometry (MS) detection. separation neutral oligosaccharides requires covalent attachment charged species electrophoretic migration. Among labels which have shown promise assisting and HPLC is fluorophore 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS). This report describes qualitative profiling ANTS-derivatized underivatized complex glycans electrospray ion trap Several standard including maltooligosaccharide ladder were ANTS subjected CE/UV CE/MS low pH buffers consisting citric 6-aminocaproic salts. detected negative ions, multiple stage MS provided valuable structural information. Fragment ions easily identified, showing identification unknowns. N-Linked from bovine fetuin used demonstrate applicability derivatization followed negatively glycans. Analyses on both species, sialylated separated forms. ability spectrometer perform was exploited, MS5 information obtained selected technique presents complementary method existing methodologies glycan mixtures. Copyright © 2002 John Wiley & Sons, Ltd.