DNA-instructed acyl transfer reactions for the synthesis of bioactive peptides.

摘要: We present a method which allows for the translation of nucleic acid information into output molecules that interfere with disease-related protein-protein interactions. The draws upon acid-templated reaction, in adjacent binding reactive conjugates triggers transfer an aminoacyl or peptidyl group from donating thioester-linked PNA-peptide hybrid to peptide-PNA acceptor. evaluated influence conjugate structures on reactivity and sequence specificity. DNA-triggered peptide synthesis proceeded specifically showed catalytic turnover template. affinity formed BIR3 domain X-linked inhibitor apoptosis protein (XIAP) is discussed.