An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA

摘要: Abstract Background Radial glia cells comprise the principal population of neural stem (NSC) during development. Attempts to develop reproducible radial and NSC culture methods have met with variable results, yielding non-adherent cultures or requiring addition growth factors. Recent studies demonstrated that a 2-μm patterned poly-methyl methacrylate (ln2 PMMA) grooved scaffold, by mimicking biophysical microtopographic properties embryonic niche, induces de-differentiation glial into functional cells. New method Here we describe for obtaining adherent Bergmann (BRG) cortical (CRG). The substrate is ln2 PMMA factors not required. Results Postnatal obtained from mouse cerebellum cerebral cortex grown on adopted BRG/CRG phenotype characterized bipolar shape, up-regulation progenitor markers such as nestin Sox2, down-regulation vimentin GFAP. Neurons cultured over aligned their processes those shafts, thus behavior migrating neuronal Comparison existing offers an ideal system analyzing both biochemical controlling neurogenic potential migration. Conclusions obtain immature preparations in vitro . It can be used study CNS well interactions between neurons, supports progenitors use different applications.