Inducing functional radial glia-like progenitors from cortical astrocyte cultures using micropatterned PMMA.

摘要: Radial glia cells (RGC) are multipotent progenitors that generate neurons and during CNS development, which also served as substrate for neuronal migration. After a lesion, reactive the main contributor to regenerative blockage, although some astrocytes able de-differentiate in situ into radial glia-like (RGLC), providing beneficial effects terms of recovery. Thus, identification properties potentiate ability transform RGLC response lesion might help development implantable devices improve endogenous regeneration. Here we demonstrate functional can be induced from vitro matured by using precisely-sized micropatterned PMMA grooved scaffold, without added soluble or adsorbed biochemical factors. were extremely organized aligned on 2 μm line patterned and, like their embryonic counterparts, express nestin, neuron-glial progenitor marker Pax6, proliferate, different intermediate support direct axonal growth Our results suggest introduction patterns size range RGC processes scaffolds mimic topography neural stem cell niche, driving an phenotype, thus favoring situ.