CD-Spectroscopy As a Powerful Tool for Investigating the Mode of Action of Unmodified Drugs in Live Cells
作者： Lutz F. Tietze , Birgit Krewer , Felix Major , Ingrid Schuberth
DOI: 10.1021/JA902767F
关键词:
摘要: Circular dichroism (CD) spectroscopy is a well-known method for the analysis of chiral chemical compounds and often used studying structure interaction proteins, DNA bioactive in solution. Here we demonstrate that CD also powerful tool investigating cellular uptake mode action drugs live cells. By means spectroscopy, identified as target several novel anticancer agents based on highly cytotoxic natural antibiotic CC-1065. Furthermore, time-dependent changes spectra drug-treated cells enabled us to rationalize differences drug cytotoxicity. The rapidly penetrate cell membrane bind their intracellular target. Thereby, formation reversible noncovalent complex with followed by covalent binding more toxic show higher stability lower alkylation rate. Since no manipulation i...
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