American families with Crohn's disease have strong evidence for linkage to chromosome 16 but not chromosome 12

摘要: Abstract Background & Aims: Two European genome-wide screens for inflammatory bowel disease have identified two significant regions of linkage on chromosomes 16 ( IBD1 ) and 12 IBD2 with suggestive levels significance (chromosomes 3p 7q). The aim this study was to determine if there evidence these in non-Jewish Ashkenazi Jewish families multiplex Crohn's from the United States. Methods: One hundred forty-eight affected relative pairs, 34% Ashkenazim, were genotyped 10–14 highly polymorphic markers overlying each candidate region. Nonparametric multipoint two-point analyses performed. Results: Significant replication found only chromosome locus, , maximal at D16S769 (nonparametric score [NPL], 2.49; P = 0.007). Analysis by ethnicity showed stronger Ashkenazim (D16S769; NPL=2.52; 0.007) than white populations (D16S401; NPL=1.40; 0.082). There no ). Minimal extension observed 7q regions. Conclusions: American families, particularly susceptibility 16, but not 12. GASTROENTEROLOGY 1998;115:1056-1061