Enhanced Antitumor Efficacy of an Oncolytic Herpes Simplex Virus Expressing an Endostatin–Angiostatin Fusion Gene in Human Glioblastoma Stem Cell Xenografts

摘要: Viruses have demonstrated strong potential for the therapeutic targeting of glioblastoma stem cells (GSCs). In this study, use a herpes simplex virus carrying endostatin–angiostatin (VAE) as novel strategy glioblastoma-derived cancer was investigated. We isolated six stable GSC-enriched cultures from 36 human specimens and selected one GSCs lines establishing GSC-carrying orthotopic nude mouse models. The following results were obtained: (a) VAE rapidly proliferated in expressed endo–angio vitro vivo 48 h 10 d after infection, respectively; (b) compared with control gliomas treated rHSV or Endostar, subcutaneous derived showed significant reduction microvessel density treatment; (c) control, improvement observed length survival mice intracranial VAE; (d) MRI analysis that tumor volumes generated by remarkably decreased treatment controls. conclusion, oncolytic efficacy animal models an fusion gene, which enhanced antitumor most likely restricting microvasculature development.