ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY (ADCC) OF ERYTHROID AND TUMOR CELLS

摘要: Publisher Summary This chapter describes and discusses a short-term 51Cr-release ADCC assay using purified mononuclear phagocytes as effector cells against hapten-modified antibody-coated target cells. The macrophages used in this are obtained from the peritoneal of thioglycollate-induced (stimulated) BCG-immunized (activated) mice; monocytes derived human peripheral blood leukocytes (PBL), U937 macrophage-like cell line. use facilitates standardization enables one to compare cytotoxic activities an population different modified with same hapten coated antiserum. Cultured murine lines can be tumor targets. HSB line is most suitable for both New sera should titered optimal dilution determined. It mentioned that mouse anti-TNP serum effectively cells, though other raised species may more cell. Peritoneal by lavage cavity 10 ml EMEM containing U heparin/ml.