In Vitro and Ex Vivo Permeability Studies of Paclitaxel and Doxorubicin From Drug-Eluting Biodegradable Ureteral Stents
作者： Alexandre A. Barros , Carlos Oliveira , Rui L. Reis , Estevão Lima , Ana Rita C. Duarte
DOI: 10.1016/J.XPHS.2017.02.023
关键词:
摘要: A drug-eluting biodegradable ureteral stent (BUS) has been developed as a new approach for the treatment of urothelial tumors upper urinary tract cancer. In previous work, this system proven to be good carrier anticancer drugs potential effective and sustainable intravesical drug delivery system. BUS revealed reduce in 75% viability human cancer cells (T24) after 72 h contact demonstrated minimal cytotoxic effect on umbilical vein endothelial (HUVECs) which were used control. we studied permeability drugs, such paclitaxel doxorubicin, alone or released from developed. We 3 different membranes study permeability: polyethersulfone (PES) membrane, HUVECs cell monolayer, an ex vivo porcine ureter. The ureter thickness was measured (864.51 μm) histological analysis performed confirm integrity urothelium. Permeability profiles during 8 h doxorubicin. per se have shown profile expected, increasing complexity membrane permeated, decreased, with PES being more permeable tissue less permeable. molecular weight also influence each higher percentage doxorubicin (26%) lower (18%) observed across (P), diffusion (D), partition (Kd) coefficients through calculated. Finally, showed that able remain only small amount can 3% 11% estimated remains is affect not noncancer cells.
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