Human Platelet Lysate as a Replacement for Fetal Bovine Serum in Limbal Stem Cell Therapy.
作者： Kunal Suri , Hwee K. Gong , Ching Yuan , Stephen C. Kaufman
DOI: 10.3109/02713683.2015.1116586
关键词:
摘要: ABSTRACTPurpose: To evaluate the use of human platelet lysate (HPL) as an alternative supplement for limbal explant culture.Methods: Culture media were prepared using either 10% pooled HPL (PHPL), single donor HPL, or fetal bovine serum (FBS). Limbal tissues, obtained from Minnesota Lions Eye Bank, cultured in each medium on plastic plates denuded amniotic membrane (AM). Immunofluorescence staining was performed ABCG2, tumor protein p63α, and cytokeratin 3 (K3). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) used to expression ABCG2 p63. explants grown labeled with bromodeoxyuridine (BrdU) assess proliferative capacity medium. Concentration growth factors including epidermal factor (EGF), vascular endothelial (VEGF), transforming factor-β (TGF-β), derived (PDGF) PHPL compared that (HS).Results: Immunofluoresc...
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