Preservation of differentiation and clonogenic potential of human hematopoietic stem and progenitor cells during lyophilization and ambient storage

摘要: Progenitor cell therapies show great promise, but their potential for clinical applications requires improved storage and transportation. Desiccated cells stored at ambient temperature would provide economic practical advantages over approaches employing freezing subzero storage. The objectives of this study were to assess a method loading the stabilizing sugar, trehalose, into hematopoietic stem progenitor (HPC) evaluate effects subsequent freeze-drying on differentiation clonogenic potential. HPC isolated from human umbilical cord blood loaded with trehalose using an endogenous surface receptor, termed P2Z. Solution containing trehalose-loaded was placed vials, which transferred tray freeze-dryer removed during each step process Control groups these experiments freshly HPC. formed 1450+/-230 CFU-GM, 430+/-140 BFU-E, 50+/-40 CFU-GEMM per 50 microL. Compared values control cells, there no statistical difference observed end or primary drying. There gradual decrease in number CFU-GM BFU-E different temperatures secondary drying; however, significant differences CFU-GEMM. To determine stability lyophilized HPC, 4 weeks 25 degrees C dark. Cells reconstituted immediately after lyophilization produced 580+/-90 ( approximately 40%, relative unprocessed controls p<0.0001), 170+/-70 (approximately 41+/-22 82%, p = 0.4171), 28 days room 513+/-170 35%, controls, 112+/-68 26%, 36+/-17 0.2164) These studies are first document high level retention following C. This type flexible potentially permit ability ship store without need refrigeration.