Considerations for Culturing and Preserving Adult Stem Cells for Therapeutics

摘要: The field of stem-cell therapy holds immense potential to develop as a new branch medicine that can not only enhance endogenous repair processes in the human body, but also be able supplement with isolated and expanded multipotent autologous stem cells especially case diseased old patients when body is unable generate sufficient healthy cells. In order supply clinical quality cells, several traditional practices were previously used for cell culture need challenged established will closely mimic physiological conditions. This review focuses on current methods cryopreservation source serum growth factors supplied during culture, role oxygen an important regulatory factor challenges controlled laboratory conditions meet needs. Despite limitations finding safe cryopreserving purpose long-term use, limited availability supplements grow at scale, extreme challenge limit exposure these sensitive cultured vitro from naturally abundant surrounding, we discuss here efforts research groups are investing improved optimize protocols. It hope increase knowledge fine-tune open doors healing traditionally hard-to-treat disease