Detection of Tumor-Specific PTPmu in Gynecological Cancer and Patient Derived Xenografts.

摘要: Background: We developed a fluorophore-conjugated peptide agent, SBK4, that detects tumor-specific proteolyzed form of the cell adhesion molecule, PTPmu, found in tumor microenvironment. previously demonstrated its tissue specific distribution high-grade brain tumors. To extend those studies to other aggressive solid types, we assessed PTPmu/SBK4 set matched gynecologic cancer patient derived xenografts (PDXs) and primary tumors, as well limited cohort tumors from gynecological patients. PDXs isolated tissues patients have been shown yield experimentally manipulatable models replicate clinical characteristics individual patients’ In this study, biopsies were examined determine if PTPmu was present. Methods: used agent SBK4 conjugated fluorophore Texas Red (TR) label microarrays (TMAs) containing and/or PDX samples several quantified level staining with Image J. one TMA, able directly compare on same slide. Results: While normal had very little SBK4-TR staining, both higher labeling SBK4-TR. Matched endometrial ovarian cancers levels by than tissue. Conclusion: sample set, all increased compared controls. Our results indicate novel detection allow for visualization changes molecules tissue-based providing rationale further development an imaging including cancers.