A Novel Molecular Diagnostic of Glioblastomas: Detection of an Extracellular Fragment of Protein Tyrosine Phosphatase μ

摘要: We recently found that normal human brain and low-grade astrocytomas express the receptor protein tyrosine phosphatase mu (PTPµ) more invasive astrocytomas, glioblastoma multiforme (GBM), downregulate full-length PTPµ expression. Loss of expression in GBMs is due to proteolytic cleavage generates an intracellular potentially a cleaved released extracellular fragment PTPµ. Here, we identify containing domains required for PTPµ-mediated adhesion remains associated with GBM tumor tissue. hypothesized detection this would make excellent diagnostic tool localization tissue within brain. To end, generated series fluorescently tagged peptide probes bind fragment. The specifically recognize cells sections surgically resected tumors. test whether are able detect tumors vivo, were tested both mouse flank intracranial xenograft model systems. glial molecularly labeled minutes tail vein injection using Maestro FLEX In Vivo Imaging System. label was stable at least 3 hours. Together, these results indicate recognition provides novel molecular glioblastomas. Such has clear translational applications may lead improved surgical resections prognosis patients devastating disease.