14-3-3 Proteins Regulate Exonuclease 1–Dependent Processing of Stalled Replication Forks
作者： Kim Engels , Michele Giannattasio , Marco Muzi-Falconi , Massimo Lopes , Stefano Ferrari
DOI: 10.1371/JOURNAL.PGEN.1001367
关键词:
摘要: Replication fork integrity, which is essential for the maintenance of genome stability, monitored by checkpoint-mediated phosphorylation events. 14-3-3 proteins are able to bind phosphorylated and were shown play an undefined role under DNA replication stress. Exonuclease 1 (Exo1) processes stalled forks in checkpoint-defective yeast cells. We now identify as vivo interaction partners Exo1, both mammalian Yeast 14-3-3–deficient cells fail induce Mec1–dependent Exo1 hyperphosphorylation accumulate Exo1–dependent ssDNA gaps at forks, revealed electron microscopy. This leads persistent checkpoint activation exacerbated recovery defects. Moreover, using bi-dimensional electrophoresis, we show that promote progression limiting nucleotide concentrations. propose assist controlling status additional unknown targets, promoting progression, restart response
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P Szankasi, G.R. Smith, A DNA exonuclease induced during meiosis of Schizosaccharomyces pombe. Journal of Biological Chemistry. ,vol. 267, pp. 3014- 3023 ,(1992) , 10.1016/S0021-9258(19)50688-3
S. Braselmann, F. McCormick, Bcr and Raf form a complex in vivo via 14‐3‐3 proteins. The EMBO Journal. ,vol. 14, pp. 4839- 4848 ,(1995) , 10.1002/J.1460-2075.1995.TB00165.X
Maria Pia Longhese, Francisca Lottersberger, Giovanna Lucchini, Veronica Baldo, Fabio Rubert, Functions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress. Genetics. ,vol. 165, pp. 1717- 1732 ,(2003)
D. Bridges, G. B. G. Moorhead, 14-3-3 proteins: a number of functions for a numbered protein. Science Signaling. ,vol. 2004, ,(2004) , 10.1126/STKE.2422004RE10
M. M. Falconi, A. Piseri, M. Ferrari, G. Lucchini, P. Plevani, M. Foiani, De novo synthesis of budding yeast DNA polymerase alpha and POL1 transcription at the G1/S boundary are not required for entrance into S phase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 10519- 10523 ,(1993) , 10.1073/PNAS.90.22.10519
Barbara J. Thomas, Rodney Rothstein, Elevated recombination rates in transcriptionally active DNA. Cell. ,vol. 56, pp. 619- 630 ,(1989) , 10.1016/0092-8674(89)90584-9
Marco Muzi-Falconi, Giordano Liberi, Chiara Lucca, Marco Foiani, Mechanisms controlling the integrity of replicating chromosomes in budding yeast. Cell Cycle. ,vol. 2, pp. 563- 566 ,(2003) , 10.4161/CC.2.6.553
Massimo Lopes, Cecilia Cotta-Ramusino, Giordano Liberi, Marco Foiani, Branch Migrating Sister Chromatid Junctions Form at Replication Origins through Rad51/Rad52-Independent Mechanisms Molecular Cell. ,vol. 12, pp. 1499- 1510 ,(2003) , 10.1016/S1097-2765(03)00473-8
Simona Fiorani, Guendalina Mimun, Laura Caleca, Daniele Piccini, Achille Pellicioli, Characterization of the activation domain of the Rad53 checkpoint kinase Cell Cycle. ,vol. 7, pp. 493- 499 ,(2008) , 10.4161/CC.7.4.5323
P. Szankasi, G. Smith, A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction Science. ,vol. 267, pp. 1166- 1169 ,(1995) , 10.1126/SCIENCE.7855597