Production of 12-hydroxy dodecanoic acid methyl ester using a signal peptide sequence-optimized transporter AlkL and a novel monooxygenase
作者： Hee-Wang Yoo , Joonwon Kim , Mahesh D. Patil , Beom Gi Park , Sung-yeon Joo
DOI: 10.1016/J.BIORTECH.2019.121812
关键词:
摘要: Abstract In this study, a signal peptide of AlkL was replaced with other peptides to improve the soluble expression and thereby facilitate transport dodecanoic acid methyl ester (DAME) substrate into E. coli. Consequently, FadL (AlkLf) showed higher activity toward DAME. Furthermore, promoter optimization for efficient heterologous transporter AlkLf alkane monooxygenase (AlkBGT) system conducted resulted in increased ω-oxygenation AlkBGT system. Moreover, bioinformatic studies led identification novel from Pseudomonas pelagia (Pel), which exhibited 20% towards DAME as compared AlkB. Finally, construction chimeric newly identified enabled production 44.8 ± 7.5 mM 12-hydroxy (HADME) 31.8 ± 1.7 mM dodecanedioic monomethyl (DDAME) two-phase reaction
sci-hub.se 参考文章(48)
Daniel Scheps, Sumire Honda Malca, Sven M. Richter, Karoline Marisch, Bettina M. Nestl, Bernhard Hauer, Synthesis of ω‐hydroxy dodecanoic acid based on an engineered CYP153A fusion construct Microbial Biotechnology. ,vol. 6, pp. 694- 707 ,(2013) , 10.1111/1751-7915.12073
Peigang Cao, André Y. Tremblay, Marc A. Dubé, Katie Morse, Effect of Membrane Pore Size on the Performance of a Membrane Reactor for Biodiesel Production Industrial & Engineering Chemistry Research. ,vol. 46, pp. 52- 58 ,(2007) , 10.1021/IE060555O
Sumire Honda Malca, Daniel Scheps, Lisa Kühnel, Elena Venegas-Venegas, Alexander Seifert, Bettina M. Nestl, Bernhard Hauer, Bacterial CYP153A monooxygenases for the synthesis of omega-hydroxylated fatty acids Chemical Communications. ,vol. 48, pp. 5115- 5117 ,(2012) , 10.1039/C2CC18103G
Da-Hye Jung, Wonji Choi, Kwon-Young Choi, Eunok Jung, Hyungdon Yun, Romas J. Kazlauskas, Byung-Gee Kim, Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system Applied Microbiology and Biotechnology. ,vol. 97, pp. 1501- 1511 ,(2013) , 10.1007/S00253-012-4358-8
Manfred Schrewe, Anders O. Magnusson, Christian Willrodt, Bruno Bühler, Andreas Schmid, Kinetic Analysis of Terminal and Unactivated CH Bond Oxyfunctionalization in Fatty Acid Methyl Esters by Monooxygenase-Based Whole-Cell Biocatalysis Advanced Synthesis & Catalysis. ,vol. 353, pp. 3485- 3495 ,(2011) , 10.1002/ADSC.201100440
Kärt Denks, Andreas Vogt, Ilie Sachelaru, Narcis-Adrian Petriman, Renuka Kudva, Hans-Georg Koch, The Sec translocon mediated protein transport in prokaryotes and eukaryotes Molecular Membrane Biology. ,vol. 31, pp. 58- 84 ,(2014) , 10.3109/09687688.2014.907455
Thomas E. England, Olke C. Uhlenbeck, Enzymatic oligoribonucleotide synthesis with T4 RNA ligase. Biochemistry. ,vol. 17, pp. 2069- 2076 ,(1978) , 10.1021/BI00604A008
Ai-Qun Yu, Nina Kurniasih Pratomo Juwono, Susanna Su Jan Leong, Matthew Wook Chang, Production of Fatty Acid-derived valuable chemicals in synthetic microbes Frontiers in Bioengineering and Biotechnology. ,vol. 2, pp. 78- 78 ,(2014) , 10.3389/FBIOE.2014.00078
Jack Coleman, Masatoshi Inukai, Masayori Inouye, Dual functions of the signal peptide in protein transfer across the membrane Cell. ,vol. 43, pp. 351- 360 ,(1985) , 10.1016/0092-8674(85)90040-6
Dennis YC Leung, Xuan Wu, Michael Kwok Hi Leung, None, A review on biodiesel production using catalyzed transesterification Applied Energy. ,vol. 87, pp. 1083- 1095 ,(2010) , 10.1016/J.APENERGY.2009.10.006