Targeted Hybrid Capture Methods

摘要: Impressive advances in next generation sequencing have made it possible to analyze large amounts of genomic data at ever decreasing costs. Methods targeted aimed evaluating selected portions the genome can be utilized identify clinically relevant disease-associated or specific therapy-associated variants. This chapter will focus on important considerations and parameters capture-based targeting methods, describe most widely used target strategies clinical testing, compare target-based capture amplification-based capture, illustrate general applications.