Observation of miRNA Gene Expression in Zebrafish Embryos by In Situ Hybridization to MicroRNA Primary Transcripts

摘要: Abstract MicroRNAs (miRNAs) add a previously unexpected layer to the post-transcriptional regulation of protein production. Although locked nucleic acids (LNAs) reveal distribution mature miRNAs by in situ hybridization (ISH) experiments zebrafish and other organisms, high cost has restricted their use. Further, LNA probes designed recognize do not distinguish expression patterns two miRNA genes that produce same sequence. Riboprobes are substantially less expensive than LNAs, but have been used detect gene because they bind with affinity short, 22-nucleotide-long miRNAs. To solve these problems, we capitalized on fact initially transcribed into long primary transcripts (pri-mRNAs). We show here conventional digoxigenin-labeled riboprobes can embryos. tested intergenic intronic (miR-10d, miR-21, miR-27a, miR-126a, miR-126b, m...