Human Mast Cells Augment Fibroblast Proliferation by Heterotypic Cell-Cell Adhesion and Action of IL-4

摘要: Mast cells have been implicated in the pathogenesis of fibrosis because their increased number chronic inflammatory reactions. In a previous study, we had shown that human mast readily attach and form heterotypic cell-cell contacts when seeded on top fibroblast monolayers. Here, report stimulate proliferation after contact. Proliferation was measured by 5-bromo-2′-deoxyuridine or [ 3 H]thymidine uptake subconfluent monolayers attachment preincubated with mitomycin C. An 18-h coculture cell line HMC-1 doubled normal skin fibroblasts. Moreover, prepared from neonatal foreskin proliferation. The stimulatory effect dependent contact since it not transferred tissue culture supernatants cells. We hypothesized cytokines secreted Several cell-derived were tested for effects Only IL-4 able to double Additional experiments revealed that: 1) as well could be completely abrogated preincubation fibroblasts an anti-IL-4R mAb blocking ligand binding; 2) acts second signal amplifies action low doses obligatory growth factors such factor platelet-derived factor.