Preanalytical, analytical (DiaSorin LIAISON) and clinical variables potentially affecting the 25-OH vitamin D estimation.

摘要: Abstract Background Vitamin D (25-OHD) physiological functions have been expanded beyond traditional bone health, increasing the importance of its estimation in Laboratory Medicine, which renders validation available methods mandatory. Aims and We evaluated some preanalytical analytical aspects 25-OHD determination effects potentially confounding clinical variables by using DiaSorin “LIAISON 25-OH TOTAL”. Results samples were extremely stable, at least short term, without requiring special transport or storage. Precision intervals (CV%) were: within run (7–11%) total precision (8–11.5%). Mean (SD) recovery was 96 (2)%. The assay linear on dilution. Comparison with radioimmunoassay (RIA) yielded acceptable correlation (Inter-rater agreement/kappa coefficient = 0.94) equivalence interval from 6 to 55 ng/mL. a general population (N = 476, age: 60 ± 14 years, 65 males). status 25‐OHD resulted inversely related parathyroid hormone levels (r = − 0.21, p  Lower concentration observed subjects diabetes (19 ± 9 vs 14 ± 7 ng/mL, In multiple logistic regression models, aging is only significant independent risk factor for low (Odds ratio, 95% confidence intervals: 3.1, 1.3–7.3; p ≤ 0.01). Conclusions confirm LIAISON as useful tool practice. Lack vitamin common among Italian adults, appears associated several cardiovascular factors.