α1,2Fucosyltransferase increases resistance to apoptosis of rat colon carcinoma cells

摘要: Accumulation of histo-blood group antigens such as Lewis b, Y and H in colon cancer is indicative poor prognosis. It accompanied by increase alpha1,2fucosyl-transferase activity, a key enzyme for synthesis these antigens. Using model carcinoma, we previously showed that alpha1,2fucosylation increases tumorigenicity. We now show tumorigenicity inversely correlates with the cells' sensitivity to apoptosis. In addition, poorly tumorigenic REG cells independently transfected three different alpha1,2fucosyltransferase cDNAs, human FUT1, rat FTA FTB were more resistant than control apoptosis induced vitro serum deprivation. Inversely, PRO cells, spontaneously immunocompetent syngeneic animals able synthesize alpha1,2fucosylated glycans, became sensitive after transfection fragment cDNA antisense orientation. Expression dramatically enhanced their rats. However, immunodeficient animals, both alpha1,2fuco-syltransferase fully metastatic, indicating presence allowed tumor escape immune control. Taken together, results increased mediated alpha1,2fucosyl-ation associated resistance from