Antiangiogenic Effectiveness of the Urokinase Receptor-Derived Peptide UPARANT in a Model of Oxygen-Induced Retinopathy.

摘要: Purpose Pharmacologic control of neovascularization is a promising approach for the treatment retinal angiogenesis. UPARANT, an inhibitor urokinase-type plasminogen activator receptor (uPAR), inhibits VEGF-driven angiogenesis in vitro and vivo. This study investigates first time effectiveness UPARANT counteracting pathologic retina. Methods Murine fragments mouse model oxygen-induced retinopathy (OIR) were used. In mice with OIR, UPARANT-treated retinas analyzed avascular area neovascular tuft formation. Levels transcription proangiogenic factors determined. effects on blood-retinal barrier (BRB), visual function, cytoarchitecture, inflammatory markers also assessed. Human umbilical vein endothelial cells (HUVECs) chick embryo chorioallantoic membrane (CAM) which was induced by vitreous fluid from patients proliferative diabetic (PDR) Results reduced VEGF-induced fragments. decreased response, VEGF, VEGF receptor-2 activity. Transcription regulating expression reduced. restored BRB integrity, recovered loss, levels markers. Restored electroretinogram does not involve any rescue cytoarchitecture. Finally, blocked PDR fluid-induced HUVEC CAM assays. Conclusions The finding that effective against may help to establish uPAR as target retinopathies. potential application diseases further supported capacity counteract angiogenic activity fluid.