Methods and materials for expression of human plasminogen variant
作者： Deborah L. Higgins , Francis J. Castellino
DOI:
关键词:
摘要: A cleavage-resistant plasminogen molecule is provided that conveniently produced in recombinant cells by expression of a nucleic acid sequence encoding the molecule. Preferably variant with modification its two-chain cleavage site. The may be purified, acylated, complexed acylated or non-acylated fibrinolytic enzymes, and formulated into pharmaceutical compositions for use thrombolytic therapy.
lens.org 参考文章(12)
Robert M. Silverstein, Purification of plasminogen ,(1974)
Yasunori Ikeda, Bjorn A. Nexo, Eileen R. Mulvihill, Patrick J. O'hara, Suguru Suzuki, Shinji Yoshitake, Akira Hashimoto, Teruaki Yuzuriha, Tissue plasminogen activator analogs having modified growth factor domains ,(1988)
Richard Anthony Godwin Smith, John Gordon Winchester, Enzyme derivatives, and their preparation ,(1980)
Tim J. Ahern, Glenn R. Larsen, Novel thrombolytic proteins ,(1987)
Joann Whitefleet-Smith, Elliot Rosen, James McLinden, Victoria A. Ploplis, Malcolm J. Fraser, James E. Tomlinson, John W. McLean, Francis J. Castellino, Expression of human plasminogen cDNA in a baculovirus vector-infected insect cell system Archives of Biochemistry and Biophysics. ,vol. 271, pp. 390- 399 ,(1989) , 10.1016/0003-9861(89)90288-9
Donald J. Davidson, Deborah L. Higgins, Francis J. Castellino, Plasminogen activator activities of equimolar complexes of streptokinase with variant recombinant plasminogens. Biochemistry. ,vol. 29, pp. 3585- 3590 ,(1990) , 10.1021/BI00466A023
Herbert Louis Heyneker, Gordon Allen Vehar, Human tissue plasminogen activator mutants, methods and intermediates therefor, and compositions using such mutants ,(1986)
Francis J. Castellino, Deborah L. Higgins, Dna encoding human plasminogen modified at the cleavage site ,(1989)
Michael Joseph Browne, Jeffery Hugh Robinson, Arthur William Russell Tyrrell, Modified enzyme and process for its preparation ,(1986)
Gordon Allen Vehar, Michael Blaber, Herbert Louis Heyneker, Protease resistant urokinase composition, its production and use ,(1986)