Development and usage of protein microarrays for the quantitative measurement of Panton-Valentine leukocidin.

摘要: Staphylococcus aureus is a human pathogen that can harbour several genes encoding exotoxins including leukocidins. A clinically most relevant factor Panton-Valentine leukocidin (PVL) because of its association with chronic, recurrent or severe skin and soft tissue infections. In this study an antibody array was designed used to obtain overview about the in vitro PVL expression levels 266 clinical isolates MRSA as well MSSA belonging wide variety clonal complexes. For purpose, novel precipitation based method used. Unknown concentrations were determined by mapping signal intensities for spotted monoclonal antibodies calibration curves resulted from experiments known recombinant LukF-PV. In cases, one complex (CC) showed similar expressions. However, there also CCs widely varying concentrations. First analyses, on measurements, low fatal conditions are not associated PVL, such sepsis, while infections yielded higher Agr-group I IV generally produced more than agr-groups II III. The few harbouring gene toxic shock syndrome toxin (tst1) particularly level producers. these issues warrant further studies. The described herein allows rapid quantification expressed proteins collections order correlate genotypic data potential parallelisation.