Deleted in azoospermia-like enhances in vitro derived porcine germ cell formation and meiosis.

摘要: Evidence supporting that deleted in azoospermia-like (DAZL) plays a key role during gametogenesis and meiosis continues to emerge. Our study aimed determine whether overexpression of DAZL using lentiviral approach somatic stem cell germ vitro differentiation culture could enhance the formation primordial cell-like cells (PLCs) oocyte-like (OLCs). Introduction at beginning induced significantly increased Fragilis-positive PLCs, which was independent mitotic proliferation. In addition, mRNA levels markers Oct4, Stella, Vasa were also higher DAZL-transduced group suppressed when knocked down small interference RNA. At later stages differentiation, expression several genes associated with meiosis, including Scp3, Dmc1, Rec8, Stra8, determined be overexpressed, abrogated by its knockdown. Exogenous introduction protein SCP3 VASA, again reversed Although not common phenomenon system, percentage SCP3-positive displaying meiotic chromosome patterns than control, as overall OLCs generated. The factors such into cell-to-germ may provide an opportunity better understand their interactions gametogenesis, providing means generation vitro.