Studies on the development of hypothalamic regulation of the hypophysial gonadotropic activity in rats.

摘要: Development of the hypothalamic regulation gonadotrophs has been studied in rats. Pituitaries rat fetuses were cut serially at 4 micrometer coronal plane, and every 5th section was mounted stained immunohistochemically with anti-HCG serum. Sum total numbers immunoreactive cells each used as an index number pituitary. Anti-rLH, anti-rLH beta, anti-rFSH, anti-rTSH beta sera also applied additionally for staining. The serum-positive first evident on day 17.5 gestation, reacting started to appear. On 19.5, very few appeared react anti-rFSH Intraperitoneal injection LHRH induced a remarkable disappearance materials, result numerical reduction occurred cells. These phenomena noted even 18.5 gestation. Encephalectomy 16.5 daily i.p anti-LHRH serum 19.5 old carried out purpose blocking effect endogenous fetal Dense staining cytoplasm size obtained after 20.5 gestation both animal groups. findings suggest that hypothalamus may start govern virtually hypophysial rat.