Surface hydrophobicity of microparticles modulates adjuvanticity.

摘要: Polymeric microparticles are promising adjuvants and they exhibit various physicochemical characteristics that can regulate the immune response, including hydrodynamic size, morphology, surface properties, among others. Surface hydrophobicity is also a key microparticle characteristic, but how it affects adjuvanticity remains unknown. To study correlation between in-depth, we prepared poly(d,l-lactic acid) (PLA)-, poly(d,l-lactic-co-glycolic (PLGA)-, poly(monomethoxypolyethylene glycol-co-d,l-lactide) (mPEG-PLA, PELA)-based by premix membrane emulsification, which were similar in size morphology differed hydrophobicity. We then systematically evaluated their ability to induce responses vitro vivo. Increased on PLA-based greatly promoted antigen internalization into dendritic cells (DCs) as well MHC II CD86 expression DCs vitro. Similarly, vivo studies showed increased significantly elevated cytokine secretion levels splenocytes harvested from vaccinated mice. Adhesion force measurements confirmed enhanced physical interaction cell membranes, condition favorable for promoting cells. Taken together, these results indicated an important factor determines magnitude of elicited vaccination with different particulate systems.