Solubilization of the Picrotoxinin Binding Receptor from Mammalian Brain
作者： William C Davis , Maharaj K Ticku
DOI: 10.1111/J.1471-4159.1981.TB00600.X
关键词:
摘要: The binding sites for alpha-dihydropicrotoxinin (DHP), which is a ligand the picrotoxin-sensitive component at benzodiazepine-gamma-aminobutyric acid-receptor-ionophore complex, has been solubilized from rat brain, using 1% Lubrol. A new assay, involves precipitation of [3H]DHP-soluble protein complex by gamma-globulin and polyethylene glycol (PEG), followed centrifugation, described. material bound DHP to two with apparent affinities 0.038 1.85 microM. receptors was inhibited convulsant depressant drugs potencies similar those required membrane receptors. ability barbiturates inhibit both strongly suggests that may interact picrotoxin component. These data suggest recognition properties picrotoxinin are not altered solubilization. abolished urea partially destroyed heating soluble extract 65 degrees C 30 min. This method measuring ligands PEG centrifugation might be used successfully in other solubilization studies.
sci-hub.se 参考文章(25)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
K. Krnjević, Chemical Nature of Synaptic Transmission in Vertebrates Physiological Reviews. ,vol. 54, pp. 418- 540 ,(1974) , 10.1152/PHYSREV.1974.54.2.418
RICHARD F. SQUIRES, CLAUS BRAESTRUP, Benzodiazepine receptors in rat brain Nature. ,vol. 266, pp. 732- 734 ,(1977) , 10.1038/266732A0
Bethan Lang, Eric A. Barnard, Li-Ren Chang, J.Oliver Dolly, Putative benzodiazepine receptor: A protein solubilised from brain FEBS Letters. ,vol. 104, pp. 149- 153 ,(1979) , 10.1016/0014-5793(79)81103-5
ROBERT N. MCBURNEY, JEFFERY L. BARKER, GABA-induced conductance fluctuations in cultured spinal neurones. Nature. ,vol. 274, pp. 596- 597 ,(1978) , 10.1038/274596A0
W.C. Davis, M.K. Ticku, Solubilization of the dihydropicrotoxinin binding sites European Journal of Pharmacology. ,vol. 68, pp. 397- 399 ,(1980) , 10.1016/0014-2999(80)90545-2
Maharaj K. Ticku, Richard W. Olsen, Interaction of barbiturates with dihydropicrotoxinin binding sites related to the GABA receptor-ionophore system Life Sciences. ,vol. 22, pp. 1643- 1651 ,(1978) , 10.1016/0024-3205(78)90061-9
N. G. BOWERY, A. DRAY, Barbiturate reversal of amino acid antagonism produced by convulsant agents. Nature. ,vol. 264, pp. 276- 278 ,(1976) , 10.1038/264276A0
M.K. Ticku, Is the picrotoxinin binding site at the GABA synapse a site of action for barbiturates Brain Research Bulletin. ,vol. 5, pp. 919- 923 ,(1980) , 10.1016/0361-9230(80)90155-0
Donald V. Greenlee, Richard W. Olsen, Solubilization of gamma-aminobutyric acid receptor protein from mammalian brain. Biochemical and Biophysical Research Communications. ,vol. 88, pp. 380- 387 ,(1979) , 10.1016/0006-291X(79)92059-X