Expression and excretion of polypeptides in eucaryotes under the control of an adenovirus promoter
作者： Pierre Tiollais , Massimo Levrero , Annick Ballay , Michel Perricaudet
DOI:
关键词:
摘要: Recombinant DNA modified by a nucleotide sequence coding for specific polypeptide whose expression is sought, this recombinant being suitable the transformation of eukaryot, especially human or animal, cell lines endogenic polymerases are capable recognising adenovirus promoters. The according to invention more particularly characterised in that said insertion placed under direct control early promoter E1A region genome.
lens.org参考文章(5)
Renato Dulbecco, Viruses with recombinant surface proteins. ,(1983)
Paolo Sassone-Corsi, René Hen, Emiliana Borrelli, Todd Leff, Pierre Chambon, Far upstream sequences are required for efficient transcription from the adenovirus-2 E1A transcription unit Nucleic Acids Research. ,vol. 11, pp. 8735- 8745 ,(1983) , 10.1093/NAR/11.24.8735
M. L. Michel, P. Pontisso, E. Sobczak, Y. Malpiece, R. E. Streeck, P. Tiollais, Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 7708- 7712 ,(1984) , 10.1073/PNAS.81.24.7708
I. Kruczek, W. Doerfler, Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 7586- 7590 ,(1983) , 10.1073/PNAS.80.24.7586
Der Werf Sylvie Van, Marc Girard, DNA fragments encoding for an immunogenic peptide capable of inducing in vivo synthesis of anti-polio virus antibodies ,(1983)