Characterization of wheat DArT markers: genetic and functional features

作者: Daniela Marone , Giosuè Panio , Donatella B. M. Ficco , Maria A. Russo , Pasquale De Vita

DOI: 10.1007/S00438-012-0714-8

关键词:

摘要: Diversity array technology (DArT) markers are largely used for mapping, genetic diversity, and association mapping studies. For years, they have been as anonymous genomic markers, their sequences were not known. As the of 2,000 wheat DArT clones now available, this study was designed to analyze these with bioinformatic approaches, features a subset 291 positioned on A B genomes in three durum maps. set 1,757 non-redundant identified, queries similarity searches. Analysis positions corresponding nearly identical indicates that redundancy is one factors explains clustering specific regions. Of total 1,124 (64 %) represent putatively expressed sequences, putative functions proposed more than 700 them. note, many correspond genes related disease resistance, characterized by leucine-rich repeat domains, 40 maps presented study. Finally, find syntenic regions Brachypodium rice genomes. In conclusion, analyses herein contribute explain main observed maps, short chromosome Moreover, attribution gene makes an optimal tool collinearity studies or identification candidate genes.

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