Exploring the Interactions of Ruthenium (II) Carbosilane Metallodendrimers and Precursors with Model Cell Membranes through a Dual Spin-Label Spin-Probe Technique Using EPR

摘要: Dendrimers exhibit unique interactions with cell membranes, arising from their nanometric size and high surface area. To a great extent, these define biological activity can be reported in situ by spin-labelling techniques. Schiff-base carbosilane ruthenium (II) metallodendrimers are promising antitumor agents mechanism of action yet to explore. In order study model membranes occurring at molecular level, namely cetyltrimethylammonium bromide micelles (CTAB) lecithin liposomes (LEC), electron paramagnetic resonance (EPR) was selected. Both spin probe, 4-(N,N-dimethyl-N-dodecyl)ammonium-2,2,6,6-tetramethylpiperidine-1-oxyl (CAT12), able enter the label, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) covalently attached newly synthesized heterofunctional dendrimers, were used provide complementary information on dendrimer–membrane interactions. The computer-aided EPR analysis demonstrated good agreement between results obtained for probe label experiments. points view suggested partial insertion dendrimer groups into surfactant aggregates, mainly CTAB micelles, occurrence both polar hydrophobic interactions, while dendrimer–LEC involved more groups. We found out that subtle changes structure greatly modified interacting abilities and, subsequently, anticancer activity.