Genetic and molecular analyses of mutations involved in Drosophila raf signal transduction.

摘要: We have identified dominant mutations that suppress the lethality associated with an R217-->L mutation in GTP.Ras binding region (CR1) of Drosophila raf (D-raf) serine/threonine kinase. Four intragenic and seven extragenic suppressors were recovered. Each four contains one compensatory amino acid change located either CR1 or kinase domain D-raf. The represent at least genetic loci whose effects strongly suggest they participate both sevenless EGF receptor (DER) signaling pathways. One these mutations, Su(D-raf)34B, is allele D-mek which encodes known molecule MAPK (MEK). A D83V D-MEK shown to be sufficient confer activity Su(D-raf)34B.