Global aspects of pacC regulation of pathogenicity genes in Colletotrichum gloeosporioides as revealed by transcriptome analysis.

摘要: Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. The transcription factor pacC is a regulator pH-controlled genes and essential for successful colonization. We present here the sequence assembly fruit pathogen use it to explore global regulation pathogenicity by ambient pH. assembled genome size was 54 Mb, encoding 18,456 genes. Transcriptomes wild type ΔpacC mutant were established RNA-seq explored their pH-dependent gene regulation. analysis showed that upregulates 478 downregulates 483 genes, comprising 5% fungal genome, including transporters, antioxidants, cell-wall-degrading enzymes. Interestingly, families with similar functionality are both up- downregulated pacC. Global secreted significant activation degradative enzymes at alkaline pH infection. Select from alkalizing-type C. acidifying-type Sclerotinia sclerotiorum verified quantitative reverse-transcription polymerase chain reaction different values. Knock out several pacC-activated confirmed involvement in pathogenic alkalinized surroundings. results suggest key change alkalinizing acidifying pathogens.