Patient-Derived Gastric Carcinoma Xenograft Mouse Models Faithfully Represent Human Tumor Molecular Diversity

摘要: Patient-derived cancer xenografts (PDCX) generally represent more reliable models of human disease in which to evaluate a potential drugs preclinical efficacy. However date, only few patient-derived gastric xenograft (PDGCX) have been reported. In this study, we aimed establish additional PDGCX and whether these accurately reflected the histological genetic diversities corresponding patient tumors. By engrafting fresh (GC) tissues into immune-compromised mice (SCID and/or nude mice), thirty two were established. Histological features assessed by qualified pathologist based on HE kappa value = 0.81~1). Protein expression PTEN MET also showed moderate agreement (agreement rate 78%; 0.46~0.56), while ERBB1 ERBB3 slight 59~75%; 0.18~0.19). ERBB2 positivity, FGFR2 or gene amplification was all maintained until passage 12 mice. The stability molecular profiles observed across subsequent passages within individual provides confidence utility translational significance for vivo testing personalized therapies.