Establishment and characterisation of patient-derived xenografts as paraclinical models for gastric cancer

摘要: The patient-derived xenograft (PDX) model is emerging as a promising translational platform to duplicate the characteristics of tumours. However, few studies have reported detailed histological and genomic analyses for fidelity factors affecting successful establishment gastric cancer. Here, we generated PDX tumours surgically-derived from 62 cancer patients. Fifteen models were successfully established (24.2%, 15/62) passaged maintain in immune-compromised mice. Diffuse type low tumour cell percentage negatively correlated with success rates (p = 0.005 p = 0.025, respectively), while reducing ex vivo overall procedure times positively (p = 0.003 p = 0.01, respectively). histology genetic stable over subsequent passages. Lymphoma transformation occurred five cases (33.3%, 5/15), all NOG mouse, none nude mouse. Together, present study identified Lauren classification, percentages, along times, key determinants engraftment. Furthermore, highly consistent between primary tumours, which provide realistic paraclinical models, enabling personalised development treatment options