Evaluation of the influence of green extraction solvents on the cytotoxic activities of Crinum (Amaryllidaeae) alkaloid extracts using in-vitro-in-silico approach.

摘要: Abstract Ethnopharmacological relevance The traditional use of Amaryllidaceae plants to treat many disease have been known for a very long period time. chemical analysis these has yielded diversity alkaloids with analgesic, anticholinergic, antitumor and antiviral activities. Crinum bulbispermum (Burm.f.) Milne-Redh. & Schweick in particular used by Zulu, Sotho Tswana people tumors as form chemotherapy, while Madagascar, powellii Baker Handb. was the treatment abscesses tumors. Many spawned genus will surely take part production anticancer drugs but their further clinical development is restricted limited commercial availability. An emerging area research establishment green extraction techniques different targeted compounds. Aim study Our comparative investigated possibility getting improved biological responses changing solvent better greener one. This aimed assess cytotoxic activity bulbs, when extracted solvents. Materials methods solvents Genapol X-80 (a surfactant-aided extraction), DES-3 (Choline chloride: fructose 5:2) mixture natural deep eutectic solvent) purified distilled water were bulbs. Extracts tested against two cell lines HEPG-2 HCT 116, doxorubicin positive reference. Molecular docking studies carried out illustrate binding orientations active site several molecular targets hepatic colorectal cancer. Results DES aided showed highest cytotoxicity lines, followed surfactant extracts finally aqueous extracts. There an obvious relationship between alkaloidal content antiproliferative potency Multivariate statistical analyses performed aid prediction responsible activity. alkaloid crinine high correlation coefficient value colon cancer line orthogonal projection latent structures (OPLS) model, suggesting that it could operate selective mode action on this line. In addition, lycorine had almost no anti-proliferative cells. confirmed same conclusions. Conclusion Herein, demonstrated (NADES) components solutions be chosen enhance prepared.