The effect of benzo(a)pyrene on porcine urinary bladder epithelial cells analyzed for the expression of selected genes and cellular toxicological endpoints

摘要: Consumption of tobacco products is the most relevant risk factor for development bladder cancer beside occupational contributions. In order to investigate mechanisms smoke components in carcinogenesis we have introduced a primary epithelial cell culture system derived from porcine urinary as suitable representative corresponding human tissue under physiological conditions. Two independent readouts were selected markers genotoxic events. Changes expression level several toxicologically genes should serve indicators early response, while classical endpoints monitored manifested damages. Here, present first results our study with benzo(a)pyrene (BaP) member polycyclic aromatic hydrocarbons (PAHs) found smoke. Cells treated BaP show dramatic increase CYP1A1 that appears be both indicator and contributor toxicity. Genes coding other proteins xenobiotic metabolism, signal transduction or tumor suppression moderate effects no enhancement their levels. Comet assay micronucleus test did significant, dose-dependent DNA damages aberrations after division. While these are conforming response at mRNA level, they less pronounced require rather higher dosages chemical.