作者: Lars T. van der Veken , Miriam Coccoris , Erwin Swart , J. H. Frederik Falkenburg , Ton N. Schumacher
DOI: 10.4049/JIMMUNOL.182.1.164
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摘要: The successful application of T cell-based immunotherapeutic applications depends on the availability large numbers cells with desired Ag specificity and phenotypic characteristics. Engineering TCR-transferred lymphocytes is an attractive strategy to obtain sufficient choice. However, introduction additional TCR chains into leads generation unknown specificity, due formation mixed dimers between endogenous introduced chains. such potentially autoaggressive may be prevented by using gammadelta as recipient cells, but in vivo activity TCR-engineered has not been established. In present study, we have investigated functionality TCR-transduced particular their specific proliferative capacity, reactivity, persistence, capacity mount recall responses. results demonstrate that alphabeta engineering forms a feasible generate Ag-specific effector do express dimers. view increasing concerns potential autoimmune consequences dimer formation, testing engineered clinical trials seems warranted.