PIP3 is involved in neuronal polarization and axon formation.

摘要: Recent experiments in various cell types such as mammalian neutrophils and Dictyostelium discoideum amoebae point to a key role for the lipid product of PI 3-kinase, PIP(3), determining internal polarity. In neurons, consequence elongation one neurite, axon is specified acquires its To test hypothesis that 3-kinase PIP(3) may play neuronal polarity, especially specification, we observed localization visualized by Akt-PH-GFP developing hippocampal neurons. We found accumulates tip growing processes. This accumulation inhibited addition inhibitors. Those inhibitors, consistently with process formation elongation, delay transition from stage 1 neurons 3 both elongation. Moreover, when immature neurite contacts bead coated laminin, substrate known induce growth cone followed rapid neurite. conditions, inhibitors inhibits future These results suggest involved possibly stimulating outgrowth. addition, second contacted beads, this rapidly elongates whereas first laminin-contacting stops, negative feedback mechanism other neurites.