Improved sensitivity of chromogenic substrate assays for urokinase and plasmin.

摘要: Abstract Commercially available synthetic substrates for the fibrinolytic enzymes plasmin and urokinase yield 4-nitroaniline during hydrolysis. A method is described intensifying color of this chromogen produced enzyme catalyzed amidolysis these chromogenic substrates. The liberated diazotized coupled with N-(1-Naphthyl)-ethylenediamine dihydrochloride to a colored complex absorption maximum at 545 nm molar absorbancy 4.54 × 10 4 . This contrasts an 380 405 only 9850 4-nitroaniline. When combined modification assay procedure substantial increase in sensitivity can be obtained. applicable all which use as when amplification desired.