Correlation of Epstein-Barr virus and its encoded proteins with Helicobacter pylori and expression of c-met and c-myc in gastric carcinoma.

摘要: AIM: To investigate the interrelationship of Epstein-Barr virus (EBV) and EBV- encoded proteins with Helicobacter pylori (H pylori) infection expression c-met c-myc oncogene in gastric carcinoma, to explore their role carcinogenesis. METHODS: One hundred eighty-five carcinoma tissues were detected by polymerase chain reaction (PCR)-Southern blot for EBV genome situ hybridization (ISH) EBV-encoded small RNA 1 (EBER1). Gastric positive EBER1 signals was confirmed EBV-associated (EBVaGC). The status H 185 carcinomas assessed rapid urease test PCR. samples PCR defined as infection. EBVaGC matched EBV-negative (EBVnGC) examined immunohistochemistry. RT-PCR Southern used detect nuclear antigens (EBNAs) 2, latent membrane protein (LMP) 1, early genes BARF1 BHRF1 cases. RESULTS: rate 59.45% (110/185) 7.03% (13/185) respectively. No difference found sex, age, pathological differentiation, clinical stages lymph node metastasis between pylori-positive pylori-negative carcinomas. However, antrum higher than that cardia body In our series, stages, location cancer not different EBVnGC EBVaGC, while male patients significantly female patients. positivity 46.15% (6/13) 81.40%(104/172) There no significant correlation overexpression group group. did show two groups. Transcripts EBNA1 all 13 EBVaGCs, both EBNA2 LMP1 mRNA detected. Six cases exhibited transcripts 2 transcripts. CONCLUSION: EBVnGCs is but predominant location, has a tendency predominance cardia/body location. associated abnormal EBVaGC. induced LMP1. may play important roles tumorigenesis through pathways.