ΔNp63α expression induces loss of cell adhesion in triple-negative breast cancer cells.

摘要: p63, a member of the p53 protein family, plays key roles in epithelial development and carcinogenesis. In breast cancer, p63 expression has been found predominantly basal-A (epithelial-type) triple-negative carcinomas (TNBC). To investigate functional role TNBC, we created MDA-MB-468 cell lines with inducible two major N-terminal isoforms, TAp63α ∆Np63α. did not have significant effect on gene profile phenotype, whilst main ΔNp63α was reduction adhesion. Gene profiling revealed genes involved adhesion migration whose relies overexpression ΔNp63α. Reduced also led to decreased proliferation vitro vivo. Similar data were obtained another line, BT-20, but BT-549 basal-B (mesenchymal-like) TNBC cells. cells, ∆Np63α much stronger effects than TAp63α. Although is mentioned mostly connection differentiation stem regulation, showed that regulation adhesion, process important metastasis invasion tumour That this seen mesenchymal-type cells suggests lineage-dependent functions, mirroring primary human cancers.