Regulation of spermatogenesis and reproductive capacity by Igf3 in tilapia.

摘要: A novel insulin-like growth factor (igf3), which is exclusively expressed in the gonads, has been widely identified fish species. Recent studies have indicated that Igf3 regulates spermatogonia proliferation and differentiation zebrafish; however, detailed information on role of this Igf needs further vivo investigation. Here, using Nile tilapia (Oreochromis niloticus) as an animal model, we report igf3 required for spermatogenesis reproduction. Knockout by CRISPR/Cas9 severely inhibited spermatogonial at 90 days after hatching, time critical meiosis initiation, resulted less spermatocytes mutants. Although continued to occur later, more spermatids were observed igf3−/− testes when compared with wild type adults, indicating spermatocyte spermatid transition. Importantly, a significantly increased occurrence apoptosis was loss Igf3. Therefore, males subfertile drastically reduced semen volume sperm count. Conversely, overexpression XY enhanced leading Transcriptomic analysis revealed absence dysregulation many genes involved cell cycle, pluripotency regulators are spermatogenesis. In addition, vitro gonadal culture 17α-methyltetosterone (MT) 11-ketotestosterone (11-KT) administration knockout cyp11c1 demonstrated expression regulated androgens, suggesting acts downstream androgens Notably, did not affect body growth, specifically functions Taken together, our data provide genetic evidence regulation reproductive capacity controlling