Cell proliferation parameters and apoptosis indices in pituitary macroadenomas

摘要: Background: Pituitary adenomas are usually well-differentiated tumors but may show locally aggressive behavior. Aim: To investigate the relationship between proliferation and apoptosis parameters tumor recurrence in a series of 20 radically resected pituitary macroadenomas (11 functioning, 9 non-functioning). Materials methods: Proliferative activity DNA ploidy were analyzed by flow cytometry (FCM) on fresh surgical specimens. Immunohisto-chemistry for Ki-67/MIB-1 anti-apoptotic protein Bcl-2 was performed paraffin-embedded specimens from same tumors. Tumor regrowth evaluated magnetic resonance imaging (MRI). Results: Six recurred after surgery, regardless hormonal hypersecretion. Pre-surgical size significantly higher recurrent than non-recurrent (p=0.003). MRI demonstrated cavernous sinus (CS) invasiveness all tumors, while none non-invasive (p=0.042, Fisher’s exact test). The content aneuploid 5/20 adenomas, one which recurred. Cell percentages S (%SPF) G2+M (%G2-M) phases proliferative index (PI) (PI= %SPF + %G2-M) diploid (p<0.05), no significant differences concerning FCM observed adenomas. Similarly, MIB-1 did not difference expression (p=0.33). immunoreactivity detected 12/15 involving 63±35% cells, recurrence. Conclusions: In this group macroadenomas, neuroradiological finding CS — nor is useful predicting