Gastrodin stimulates anticancer immune response and represses transplanted H22 hepatic ascitic tumor cell growth: Involvement of NF-κB signaling activation in CD4+ T cells

摘要: Gastrodia elata Blume (G. elata) is a famous restorative food in East Asia. It can be used as an auxiliary reagent hepatocellular carcinoma (HCC) treatment. Previous studies unveiled that G. exhibited immunomodulatory activities. To explore the active ingredients contributing to its activities, gastrodin, vanillin, and parishin B were purified from their anti-HCC effects assessed vivo. Among these compounds, only gastrodin was capable of repressing transplanted H22 ascitic hepatic tumor cell growth vivo with low toxicity. Further investigations designed on immune system tumor-bearing mice potential molecular mechanisms underlying effects. Our data showed ameliorated transplantation-induced activation endogenous pro-apoptotic pathway CD4+ T cells abnormalities serum cytokine profiles host animals. These events enhanced cytotoxic activities natural killer CD8+ against cancer cells. Gastrodin administration specifically upregulated mRNA levels several nuclear factor κB (NF-κB) responsive genes but not Chromatin immunoprecipitation assay increased association NF-κB p65 subunit promoter regions IL-2 Bcl-2 encoding demonstrated main ingredient anticancer properties elata. Promoting NF-κB-mediated gene transcription implicated activity.