Digging deeper into the plant cell wall proteome.

摘要: The proteome of the plant cell wall/apoplast is less well characterized than those other subcellular compartments. This largely reflects many technical challenges involved in extracting and identifying extracellular proteins, which resist isolation identification, capturing a population that both comprehensive relatively uncontaminated with intracellular proteins. However, range disruptive techniques, involving tissue homogenization subsequent sequential extraction non-disruptive approaches has been developed. These have complemented more recently by genome-scale screens, such as secretion traps reveal genes encoding proteins N-terminal signal peptides are targeted to secretory pathway, subsequently localized wall. While size complexity wall still unresolved, combination experimental tools computational prediction rapidly expanding catalog known wall-localized suggesting unexpected localization polypeptides providing basis for further exploration structure function.