Effects of glucocorticoids on human brown adipocytes.

摘要: Clinical cases of glucocorticoid (GC) excess are characterized by increased fat mass and obesity through the accumulation white adipocytes. The effects GCs on growth function brown adipose tissue unknown may contribute to negative energy balance observed clinically. This study aims evaluate effect proliferation, differentiation, metabolic Human adipocytes sourced from supraclavicular biopsies were grown in culture differentiated mature subcutaneous abdominal cultured as controls. Effects dexamethasone growth, differentiation (UCP1, CIDEA, PPARGC1A expression), (oxygen consumption rate (OCR)) quantified. Dexamethasone (1 μM) significantly stimulated proliferation preadipocytes reduced that preadipocytes. During (at 0.1, 1, 10 expression UCP1, a concentration-dependent manner enhanced fourfold sixfold OCR Isoprenaline (100 nM) (P<0.05) UCP1 These dexamethasone. Thus, we show stimulates human but inhibits adrenergic stimulation functioning We conclude exert complex development findings provide strong evidence for an biology (BAT) involvement BAT system manifestation Cushing's syndrome.